| Ouchterlony technique | A technique in which both reaction partners (antigen and antibody) are allowed to diffuse to each other in a gel in a precipitation reaction. (05 Mar 2000) |
|---|
| Ouchterlony | Orjan, Swedish bacteriologist, *1914. See: Ouchterlony method, Ouchterlony technique, Ouchterlony test, Ouchterlony technique. (05 Mar 2000) |
|---|---|
| Ouchterlony assay | <investigation> Immunological test for antigen antibody reactions in which diffusion of soluble antigen and antibody in a gel leads to precipitation of an antigen-antibody complex, visible usually as a whitish band. The system has the advantage that, because of radial diffusion of the reagents, a very wide range of ratios of antigen to antibody concentration develop, thus it is likely that precipitation will occur somewhere in the gel even when no care is taken with quantitation of the system. (18 Nov 1997) |
| Ouchterlony method | Double (gel) diffusion test in two dimensions. See: gel diffusion precipitin tests in two dimensions. Synonym: Ouchterlony method. (05 Mar 2000) |
| Ouchterlony test | Double (gel) diffusion test in two dimensions. See: gel diffusion precipitin tests in two dimensions. Synonym: Ouchterlony method. (05 Mar 2000) |
| airbrasive technique | A method of grinding, cutting tooth structure, or roughening the natural tooth surface or the surface of a restoration, by means of a device utilizing a gas-impelled jet of fine Al203 particles which, after striking the tooth, are removed by an aspirator. See: microetching technique. (05 Mar 2000) |
| air-gap technique | Chest radiography performed using a space between the subject and film instead of a grid to absorb scattered radiation; usually requires a target-film distance of 10 feet. (05 Mar 2000) |
| aseptic technique | <procedure> A method used by microbiologists and clinicians to keep cultures, sterile instruments and media, and people free of microbial contamination. (09 Oct 1997) |
| atrial-well technique | An obsolete semi-closed surgical technique for repairing atrial septal defects and other cardiac abnormalities. (05 Mar 2000) |
| Barcroft-Warburg technique | An apparatus for measuring the oxygen consumption of incubated tissue slices by manometric measurement of changes in gas pressure produced by oxygen absorption in an enclosed flask. Synonym: Barcroft-Warburg apparatus. (05 Mar 2000) |
| Begg light wire differential force technique | An orthodontic appliance utilizing small gauge labial wires with expansion and contraction loops formed into it and attached to bands fitted to individual teeth; sometimes called Begg light wire differential force technique. (05 Mar 2000) |
| Berk Sharp technique | <molecular biology, procedure> A technique of genetic mapping in which mRNA is hybridised with single stranded DNA and the nonhybridised DNA then digested with S1 nuclease, the residual DNA that hybridised with the messenger is then characterised by restriction mapping. (18 Nov 1997) |
| bone demineralization technique | Removal of mineral constituents or salts from bone or bone tissue. Demineralization is used as a method of studying bone strength and bone chemistry. (12 Dec 1998) |
| radioisotope dilution technique | Method for assessing flow through a system by injection of a known quantity of radionuclide into the system and monitoring its concentration over time at a specific point in the system. (12 Dec 1998) |
| random amplified polymorphic DNA technique | Technique that utilises low-stringency polymerase chain reaction (pcr) amplification with single primers of arbitrary sequence to generate strain-specific arrays of anonymous DNA fragments. Rapd technique may be used to determine taxonomic identity, assess kinship relationships, analyze mixed genome samples, and create specific probes. (12 Dec 1998) |
| PAP technique | 1. <gynaecology> Colloquial abbreviation for Papanicolaou's stain. 2. <technique> Peroxidase antiperoxidase method for obtaining an enhanced peroxidase reaction to indicate antibody binding to antigen. In the first stage the material, for example a section, is reacted with a specific antiserum (say rat) against the antigen. In the next stage a large excess of say rabbit antirat immunoglobulin is applied so that only one of the binding sites is bound to the first antibody. Then a rat antiperoxidase antiserum is bound to the second antibody unfilled sites and finally peroxidase is added and binds to the third antiserum before the peroxidase is used to develop a colour reaction. (18 Nov 1997) |